SolisFAST® SolisGreen® qPCR Mix
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SolisFAST® SolisGreen® qPCR Mix is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.
Concentration: 5x Hot-start: yes, 3 minutes initial incubation at 95°C Cycling speed: fast and ultra-fast Detection type: dye-based, includes SolisGreen® intercalating dye Reference dye: based on ROX Compatible real-time instruments: The mix is compatible with qPCR cyclers where ROX is used as a passive reference signal for normalization of the data. Check Your cycler! Mix Components: SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel SolisGreen® dye This dye-based qPCR mix contains next generation DNA binding dye SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major realtime cyclers. SolisGreen® dye shows: high fluorescence level high sensitivity for detecting low template concentrations high stability for room temperature storage